Antibody Services
Sangon provides a one-stop solution for worldwide customers' antibody needs. Our antibody production services include recombinant protein expression, peptide design and synthesis, polyclonal and monoclonal antibody production and affinity purification. Our packages provide you desired antibodies that will be successful in basic applications or guaranteed to work in your assay of choice.
Advantages:
- Stable and reliable technology: we provide exceptional service at any time;
- Professional peptide design and synthesis protocols;
- Superior quality with very competitive prices;
- Guaranteed services.
Antibody Service Flowchart:
Polyclonal antibody production services:
| Description | Service Protocol | Deliverables | Guarantee | Price |
| Polyclonal Standard Peptide Package | Peptide design, synthesis and conjugation (additional fees required) | Purified antibody (at least 2mg) | ELISA titre > 1:50,000. | $475 |
| Immunize 2 New Zealand Rabbits | 1-2 ml of pre-immune serum | |||
| Serum collection | 1 mg peptide | |||
| Antigen affinity purification | Progress reports | |||
| Antibody validation | QC data | |||
| Polyclonal Guaranteed Peptide Package | Peptide design, synthesis and conjugation (additional fees required) | 1st shipment: sample antibody | Dot Blot-positive against peptide and have ELISA titre > 1:50,000. Works in Customer Assay. | $1350 Set up of $400 |
| Immunize 4 New Zealand Rabbits | 2nd shipment: purified antibody (at least 2mg) | |||
| Serum collection | 1-2 ml of pre-immune serum | |||
| Antigen affinity purification | 1 mg peptide | |||
| Progress reports | ||||
| Antibody validation | QC data | |||
| Polyclonal Standard Protein Package | QC client's antigen. If DNA is provided, additional fee is required for expression. | Purified antibody (at least 2mg) | ELISA titre > 1:50,000. | $475 |
| Immunize 2 New Zealand Rabbits | ||||
| Serum collection | If protein is expressed by Sangon, 50ug -500ug of antigen will be provided. | |||
| Protein A affinity purification | 1-2 ml of pre-immune serum | |||
| Antibody validation | Progress reports | |||
| QC data | ||||
| Polyclonal Guaranteed Protein Package | QC client's antigen. If DNA is provided, additional fee is required for expression. | 1st shipment: sample antibody | Dot Blot-positive against protein and have ELISA titre > 1:50,000. Works in Customer Assay. | $1350 Set up of $400 |
| Immunize 4 New Zealand Rabbits | ||||
| Serum collection | 2nd shipment: purified antibody (at least 2mg) | |||
| Antigen affinity purification | If protein is expressed by Sangon, 50ug -500ug of antigen will be provided. | |||
| 1-2 ml of pre-immune serum | ||||
| Antibody validation | Progress reports | |||
| QC data | ||||
| Polyclonal Phospho-Specific Package | Peptide design, synthesis and conjugation, including the modified peptide and unmodified control peptide (additional fee required). | Purified antibody (at least 1mg) | Dot blot-positive against peptide and have ELISA titre > 1:50,000, and modified/unmodified titer ratio > 8. | $1900 Set up of $600 |
| Immunize 5 New Zealand Rabbits | ||||
| Serum collection | ||||
| Antigen affinity purification and cross-reactivity reduction | 1 mg modified peptide and 1 mg unmodified peptide | |||
| 1-2 ml of pre-immune serum | ||||
| Antibody validation | Progress reports | |||
| QC data | ||||
Monoclonal antibody production services:
| Description | Service protocol | Deliverables | Guarantee | Price |
| Monoclonal Standard Package | Antigen design and production or QC client's antigen | 1-3 hybridoma cell lines | ELISA titer > 1:50,000 | $2850 Set up of $850 |
| Immunize 5 Balb/c mice | 2 mg purified antibody from 1 hybridoma cell line | |||
| Fusion and ELISA screening | 1 mg peptide or 50ug -500ug of protein if expressed by Sangon | |||
| Subcloning (2-4 rounds) | 50-100ul of pre-immune serum(1:10 dilution) | |||
| Antibody production | Progress reports | |||
| Protein A/G affinity purification | QC data | |||
| Antibody validation | ||||
| Monoclonal Guaranteed Package | Antigen design and production or QC client's antigen | 1st shipment: Suerpernant from 10 ELISA-positive hybridoma cell lines | 10 ELISA-positive clones | $4250 Set up of $1,200 |
| 1 clone works in customer assay | ||||
| 2nd shipment: 1 hybridoma cell line with 2 mg purified antibody | ||||
| Immunize 10 Balb/c mice | 1 mg peptide or 50ug -500ug of protein if expressed by Sangon | |||
| Fusion and ELISA screening | 50-100ul of pre-immune serum(1:10 dilution) | |||
| Subcloning (2-4 rounds) | Progress reports | |||
| Protein A/G affinity purification | QC data | |||
| Antibody validation | ||||
| Monoclonal Phospho-Specific Package | Peptide design, synthesis and conjugation, including the modified peptide and unmodified control peptide (additional fee required) | 1 hybridoma cell line with 2 mg purified antibody | 1 clone that is Dot Blot positive | $5100 Set up of $1,500 |
| ELISA titre > 1:50,000 | ||||
| Immunize 10 Balb/c mice | 1 mg modified peptide and 1 mg unmodified peptide | Modified/unmodified titer ratio > 8 | ||
| Fusion and ELISA screening | 50-100ul of pre-immune serum(1:10 dilution) | |||
| Progress reports | ||||
| Subcloning (2-4 rounds) | QC data | |||
| Antibody production | ||||
| Protein A/G affinity purification | ||||
| Antibody validation |
More Information
How To Order
Download Antibody Order Form and email to antibody@sangon.com and copy orders to fukang@sangon.com