Flyer for Hipure&GMPstd oligo-sent-edit
1) Except for COVID-19, we offer a very attractive promotion for new customers.
Clone Gene
| ≤700 bp | 701-3000 bp | >3000 bp | Cloning fee |
| $ 70.00 | $ 0.10/bp | inquiry | $ 30.00 |
Gene Fragment
| ≤750 bp | 751 bp-1000 bp | 1001 bp-1250 bp |
| $ 65.00 | $ 90.00 | $ 120.00 |
Note: Sangon clone vector is free and cloning free is only for
customized vector.
2) SARS-CoV-2 Plasmid
Below are plasmids in stock and can be delivered around 1-2 days.
Sequences are
attached in file.
| Gene name | Length (bp) | MW | Copies/μg | Package | Vector | Price ($) |
| CoV orf3 | 3,548 | 2,192,243 | 2.746×10^11 | 2ug/tube | PUC57 | 35.00 |
| CoV E | 2,948 | 1,821,562 | 3.305×10^11 | 2ug/tube | PUC57 | 35.00 |
| CoV M | 3,389 | 2,094,034 | 2.875×10^11 | 2ug/tube | PUC57 | 35.00 |
| CoV orf6 | 2,906 | 1,795,596 | 3.353×10^11 | 2ug/tube | PUC57 | 35.00 |
| CoV orf7 | 3,086 | 1,906,816 | 3.157×10^11 | 2ug/tube | PUC57 | 35.00 |
| CoV orf8 | 2,906 | 1,795,596 | 3.353×10^11 | 2ug/tube | PUC57 | 35.00 |
| CDC V2 | 2,983 | 1,843,211 | 3.266×10^11 | 4ug/tube | PUC57 | 35.00 |
| WHO-orf1ab | 2,814 | 1,738,786 | 3.462×10^11 | 2ug/tube | pUC-SP | 20.00 |
| WHO-E | 2,826 | 1,746,199 | 3.447×10^11 | 2ug/tube | pUC-SP | 20.00 |
| CoV N | 3,970 | 2,453,047 | 2.454×10^11 | 4ug/tube | PUC57 | 80.00 |
| CoV S | 6,532 | 4,035,666 | 1.492×10^11 | 2ug/tube | pUC57 | 140.00 |
3) We produced enough stock for both SARS-CoV-2 related primers and probes, in order to help diagnostic and research industry to find out COVID-19 precisely and quickly.
| Resources | Information | Primer Name | Sequence (5' to 3') | Length | Modification |
| CDC | RRF1a/b qPCR primer and probes | ORF1ab-F | CCCTGTGGGTTTTACACTTAA | 21 | |
| ORF1ab-R | ACGATTGTGCATCAGCTGA | 19 | |||
| ORF1ab-P | CCGTCTGCGGTATGTGGAAAGGTTATGG | 28 | 5`-FAM，3`BHQ1 | ||
| N qPCR primer and probes | N-F | GGGGAACTTCTCCTGCTAGAAT | 22 | ||
| N-R | CAGACATTTTGCTCTCAAGCTG | 22 | |||
| N-P | TTGCTGCTGCTTGACAGATT | 20 | 5`-FAM，3`TAMRA | ||
| WHO | RdRP_SARSr P2 qPCR primer and probes | RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG | 22 | |
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 26 | |||
| RdRP_SARSr-P2 | CAGGTGGAACCTCATCAGGAGATGC | 25 | 5`-FAM，3`BHQ1 | ||
| RdRP_SARSr P1 qPCR primer and probes | RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG | 22 | ||
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 26 | |||
| RdRP_SARSr-P1 | CCAGGTGGWACRTCATCMGGTGATGC | 26 | 5`-FAM，3`TAMRA | ||
| E_Sarbeco qPCR primer and probes | E_Sarbeco_F1 | ACAGGTACGTTAATAGTTAATAGCGT | 26 | ||
| E_Sarbeco_R2 | ATATTGCAGCAGTACGCACACA | 22 | |||
| E_Sarbeco_P1 | ACACTAGCCATCCTTACTGCGCTTCG | 26 | 5`-FAM，3`BHQ1 |
Note:
1. References from CDC: http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html
2. References from WHO, Diagnostic detection of 2019-nCoV by real-time RT-PCR (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2), RdRP_SARSr-P2 is the specific probe for 2019-ConV, while P1 is universal probe for 2019-nCoV, SARS-CoV and bat-SARS-related CoV.
1. References from CDC: http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html
2. References from WHO, Diagnostic detection of 2019-nCoV by real-time RT-PCR (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2), RdRP_SARSr-P2 is the specific probe for 2019-ConV, while P1 is universal probe for 2019-nCoV, SARS-CoV and bat-SARS-related CoV.
The above details contain published sequences by CDC and WHO and the
package is 1 OD per tube. If you need customized package, please
make note.
4) How to Order
Please send your order to
order@sangon.com
and contact number is +86 21 57072105