SEQUENCING PRICE LIST
| Section | Name | Size | Distributor Price |
| SEQ-1 | Bacteria/Plasmids/PCR | 1-9 samples | $4.50 |
| SEQ-10 | Bacteria/Plasmids/PCR | 10-49 samples | $ 4.30 |
| SEQ-50 | Bacteria/Plasmids/PCR | 50-99 samples | $ 4.00 |
| SEQ-100 | Bacteria/Plasmids/PCR | 100-499 samples | $ 3.60 |
| SEQ-500 | Bacteria/Plasmids/PCR | over 500 samples | $ 3.00 |
| SEQ-Bulk | Bacteria/Plasmids/PCR | Inquire | |
| SEQ-Oligo | Primer Synthesis (3OD HAP purification) | $0.16/base |
Free Primers:
| Universal Primer | Sequence |
| 27F | AGAGTTTGATCMTGGCTCAG |
| 1492r | GGTTACCTTGTTACGACTT |
| 8f | AGAGTTTGATCCTGGCTCAG |
| 1541r | AAGGAGGTGATCCAGCC |
| ITS1 | TCCGTAGGTGAACCTGCGG |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG |
| ITS4 | TCCTCCGCTTATTGATATGC |
| -96III | CCCTCATAGTTAGCGTAACG |
| 5’AD | AATACCACTACAATggATgATg |
| 3’AD | gAgATggTgCACgATgCACAgT |
| 5’BD: | GTGCGACATCATCATCGGAAG |
| 3’BD | TAAGAGTCACTTTAAAATTTGTATC |
| 5’AOX | CTggTTCCAATTgACAAgC |
| 3’AOX | TggCATTCTgACATCCTC |
| a-factor | TACTATTgCCAgCATTgCTgC |
| BGH | CAgggTCAAggAAggCAC |
| CMV-F | CGCAAATGGGCGGTAGGCGTG |
| CMV-R | TCGTTGGGCGGTCAGC |
| CMV-24 | TATTAGGACAAGGCTGGTGGGCAC |
| CMV-30 | AATGTCGTAATAACCCCGCCCCGTTGACGC |
| M13(-47) | AgggTTTTCCCAgTCACg |
| M13(-48) | gAgCggATAACAATTTCACAC |
| M13F(-20) | gTTgTAAAACgACggCCAg |
| M13R(-26) | CAggAAACAgCTATgAC |
| M13-40 | GTCGTGGACTGGGAAAAC |
| M13-D | Agg gTT TTC CCA gTC Acg |
| RV-M | gAg Cgg ATA ACA ATT TCA CAC |
| PBV220+ | gggCAgCATTCAAAgCAg |
| PBV220- | TTATCAgACCgCTTCTgC |
| pCMV5-F | TCTAAAAgCTgCggAATTgT-3’ |
| pCMV5-R | TCCAAACTCATCAATgTATC-3’ |
| pEGFP-N5’ | CggTgggAggTCTATATAAG |
| pEGFP-N3’ | gTCgCCgTCCAgCTCgACCAg |
| pEGEP-C5 | CATggTCCTgCTGGAgTTCgT |
| pEGEP-C3’ | TATgTTTCAggTTCAggg |
| pGEX5’ | gCAAgCCACgTTTggTg |
| PGEX3 | gAgCTgCATgTgTCAgAgg |
| pGL3+ | TAgCAAAATAggCTgTCCC |
| pGL3- | CTTTATGTTTTTGGCGTCTTC |
| pQE30+ | gTgAgCggATAACAATTTCAC |
| pQE30- | CTgAACAAATCCAgATggAg |
| PT5 | TCCgAgATCTggACgAgC |
| SP6 | ATT TAg gTg ACA CTA TAg AA |
| S-TAG | CGAACGCCAGCACATGGACA |
| T7 | TAA TAC gAC TCA CTA TAg g |
| T3 | ATT AAC CCT CAC TAA Agg |
| T7-TER | gCTAgTTATTgCTCAgCgg |
DNA cloning and Sequencing
| Section | Name | Size | Distributor Price | |
| Clone-1 | PCR Product Clone | 1-9 samples | $ 25.00 | Not including the sequencing price |
| Clone-10 | PCR Product Clone | 10-49 samples | $ 20.00 | Not including the sequencing price |
| Clone-50 | PCR Product Clone | over 50 samples | $ 15.00 | Not including the sequencing price |
| Clone-others | The other clones | Inquire | Inquire |
Note:
the above experimental expense is for DNA fragment length less than 2 kb situation developed, if DNA fragment length more than 2 kb or the sequencing is needed after cloning, specific prices please contact.
Cosmid/Fosmid/BAC end sequencing
| Section | Name | Size | time(working day) | Distributor Price |
| BAC P-1 | BAC Plasmids | 1-9 samples | 3 | $ 12.00 |
| BAC P-10 | 10-49 samples | $ 10.00 | ||
| BAC P-50 | over 50 samples | $ 8.00 | ||
| BAC B-1 | BAC Bacteria | 1-9 samples | 5 | $ 15.00 |
| BAC B-10 | 10-49 samples | $ 12.00 | ||
| BAC B-50 | over 50 samples | $ 10.00 | ||
| Cos/Fos P-1 | Cosmid/FosmidPlasmids | 1-9 samples | 3 | $ 8.00 |
| Cos/Fos P-10 | 10-49 samples | $ 6.00 | ||
| Cos/Fos P-50 | over 50 samples | $ 5.00 | ||
| Cos/Fos B-1 | Cosmid/FosmidBacteria | 1-9 samples | 5 | $ 12.00 |
| Cos/Fos B-10 | 10-49 samples | $ 10.00 | ||
| Cos/Fos B-50 | over 50 samples | $ 8.00 |
Shotgun sequencing
| Section | Name | time(working day) | Distributor Price($/kb) |
| Shotgun-1 | BAC/Cosmid/Fosmid | 30 | $ 60.00 |
| Shotgun-2 | Chloroplast / mitochondria / virus / phage | 45 | $ 60.00 |
| Shotgun-3* | Other large fragment | 30 | $ 60.00 |
Note:
mark * : the least order amount reached $750.00