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Flyer for Hipure&GMPstd oligo-sent-edit
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Yield and Pricing
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Purification (mass QC)
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Available Lengths
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Synthesis Scale |
Yield |
Promo. Price/Base ($)
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Promo. Mod.& Pur. Cost ($)
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PAGE
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11-59bases
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100 nmole |
2 ODs |
$0.12 |
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| 200 nmole |
5 ODs |
$0.15 |
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| 400 nmole |
10 ODs |
$0.20 |
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| 1 µmole |
20 ODs |
$0.40 |
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60-69bases
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200 nmole |
2 ODs |
$0.18 |
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| 400 nmole |
5 ODs |
$0.30 |
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| 1 µmole |
10 ODs |
$0.45 |
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70-99bases
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200 nmole |
2 ODs |
$0.25 |
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| 400 nmole |
5 ODs |
$0.38 |
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| 1 µmole |
10 ODs |
$0.55 |
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100-130bases
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400 nmole |
2 ODs |
$0.45 |
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| 1 µmole |
5 ODs |
$0.60 |
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Purification (mass QC)
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Available Lengths
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Synthesis Scale |
Yield |
Promo. Price/Base ($)
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Promo. Mod.& Pur. Cost ($)
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HPLC
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5-59bases
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100 nmole |
2 ODs |
$0.12 |
$3.00 |
| 200 nmole |
5 ODs |
$0.15 |
$4.00 |
| 400 nmole |
10 ODs |
$0.20 |
$6.00 |
| 1 µmole |
20 ODs |
$0.40 |
$12.00 |
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60-69bases
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200 nmole |
2 ODs |
$0.18 |
$3.00 |
| 400 nmole |
5 ODs |
$0.30 |
$4.00 |
| 1 µmole |
10 ODs |
$0.45 |
$6.00 |
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70-99bases
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200 nmole |
2 ODs |
$0.25 |
$3.00 |
| 400 nmole |
5 ODs |
$0.38 |
$4.00 |
| 1 µmole |
10 ODs |
$0.55 |
$6.00 |
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100-130bases
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400 nmole |
2 ODs |
$0.45 |
$3.00 |
| 1 µmole |
5 ODs |
$0.60 |
$4.00 |
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Purification (mass QC)
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Available Lengths
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Synthesis Scale |
Yield |
Promo. Price/Base ($)
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Promo. Mod.& Pur. Cost ($)
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HPLC_CE
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11-59 bases
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100 nmole |
2 ODs |
$0.12 |
$8.00 |
| 200 nmole |
5 ODs |
$0.15 |
$9.00 |
| 400 nmole |
10 ODs |
$0.20 |
$11.00 |
| 1 µmole |
20 ODs |
$0.40 |
$15.00 |
| 5 µmole |
100 ODs |
$2.00 |
$50.00 |
| 10 µmole |
200 ODs |
$4.00 |
$90.00 |
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Sangon has more than 200 modified products, including Attachment
Chemistry & Linkers, Dark Quenchers, Fluorophores, Modified Bases,
Phosphorylation, Spacers, Dual-Labeled DNA Probes, Molecular
Beacons, et al., the specific list is available at
www.lifebiotech.com.
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Our Experience
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Sangong Biotech is one of the earliest and largest biotechnology
companies in China. We are a well-known supplier with comprehensive
coverage in the life sciences industry and one of the world's
largest producers of DNA synthesis. For nearly 30 years, Sangon
Biotech has been synthesizing oligonucleotides for a variety of
research applications. The number of scientific researchers wordwide
using our primers has exceeded 70,000. We provide eight purification
methods so that customers can choose the most suitable purification
method according to their actual needs. We also specializes in
modified oligonucleotides and we can provide more than 140 different
types of oligo modifications.
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Different Purification Methods
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Purification
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Introduction
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Feature
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Conventional purification methods
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HAP
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Purification by HAP resin purification column has a larger load
than OPC and avoids the carryover of short fragments, increasing
speed and purity.
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Stable quality with fast speed
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PAGE
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Polyacrylamide gel electrophoresis (PAGE) based purification.
Recommended for longer oligos. Salts removed. Majority of
failure sequences removed. MS data provided.
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Stable quality with fast speed
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ULTRAPAGE
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An upgrade of PAGE, the DNA was qualitatively analyzed with PAGE
purification and mass spectrometry to ensure the accuracy of the
recovered fragments.
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Stable quality with high rate of accuracy
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HPLC
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High Performance Liquid Chromatography (HPLC) based
purification. Recommended for shorter oligos. Salts are removed.
Majority of failure sequences are removed. MS data can be
provided.
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High degree of automation with high purity
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HPLC_CE
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HPLC with Capillary Electrophoresis. Guaranteed minimum purity
of 85% ensured. Recommended for shorter oligos. Salts are
removed. Majority of failure sequences are removed. MS data can
be provided.
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HPLC+CE with higher purity
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Additional purification methods
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IE-HPLC
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Ion exchange (IE) HPLC separates and purifies full-length
primers from truncated primers based on relative charge
difference, which can effectively remove N-1 short fragments. It
is suitable for most modifications, but not for thio, sulfhydryl
and other modifications.
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Higher purity
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2X HPLC
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Dual HPLC purification (reversed-phase, then ion exchanged),
especially suitable for modified primers longer than 59 bases
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Higher purity
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PAGE+HPLC
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For primers with high purity requirements, we offer PAGE+HPLC
dual purification, i.e. one PAGE purification followed by
another HPLC purification to further improve purity, especially
for primers longer than 59 bases with modifications
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Higher purity
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Different Purification Methods
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Purification
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Purification Method
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HAP
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PAGE
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ULTRAPAGE
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HPLC
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HPLC_CE (IVD)
IE-HPLC
2X HPLC
PAGE+HPLC
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Normal PCR/RT-PCR
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DNA sequencing
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Gene synthesis
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Multiple PCR/qPCR
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Diagnostic PCR amplification
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NGS
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Subcloning, point mutation
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Gene construction/ RNA interference
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PCR production used for gene cloning, expression or genetic recombination
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Antisense nucleic acid
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Modify or mark primers/chemical or physical applications
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IVD
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“√” means recommended modificaton methods
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Clean DNA Workshop
100% MS Detection
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DNA Drying Room
CE Detection
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