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Flyer for Hipure&GMPstd oligo-sent-edit
Yield and Pricing
Purification (mass QC) Available Lengths Synthesis Scale Yield Promo. Price/Base ($) Promo. Mod.& Pur. Cost ($)
PAGE 11-59bases 100 nmole 2 ODs $0.12
200 nmole 5 ODs $0.15
400 nmole 10 ODs $0.20
1 µmole 20 ODs $0.40
60-69bases 200 nmole 2 ODs $0.18
400 nmole 5 ODs $0.30
1 µmole 10 ODs $0.45
70-99bases 200 nmole 2 ODs $0.25
400 nmole 5 ODs $0.38
1 µmole 10 ODs $0.55
100-130bases 400 nmole 2 ODs $0.45
1 µmole 5 ODs $0.60
Purification (mass QC) Available Lengths Synthesis Scale Yield Promo. Price/Base ($) Promo. Mod.& Pur. Cost ($)
HPLC 5-59bases 100 nmole 2 ODs $0.12 $3.00
200 nmole 5 ODs $0.15 $4.00
400 nmole 10 ODs $0.20 $6.00
1 µmole 20 ODs $0.40 $12.00
60-69bases 200 nmole 2 ODs $0.18 $3.00
400 nmole 5 ODs $0.30 $4.00
1 µmole 10 ODs $0.45 $6.00
70-99bases 200 nmole 2 ODs $0.25 $3.00
400 nmole 5 ODs $0.38 $4.00
1 µmole 10 ODs $0.55 $6.00
100-130bases 400 nmole 2 ODs $0.45 $3.00
1 µmole 5 ODs $0.60 $4.00
Purification (mass QC) Available Lengths Synthesis Scale Yield Promo. Price/Base ($) Promo. Mod.& Pur. Cost ($)
HPLC_CE 11-59 bases 100 nmole 2 ODs $0.12 $8.00
200 nmole 5 ODs $0.15 $9.00
400 nmole 10 ODs $0.20 $11.00
1 µmole 20 ODs $0.40 $15.00
5 µmole 100 ODs $2.00 $50.00
10 µmole 200 ODs $4.00 $90.00

Sangon has more than 200 modified products, including Attachment Chemistry & Linkers, Dark Quenchers, Fluorophores, Modified Bases, Phosphorylation, Spacers, Dual-Labeled DNA Probes, Molecular Beacons, et al., the specific list is available at www.lifebiotech.com.

Our Experience

Sangong Biotech is one of the earliest and largest biotechnology companies in China. We are a well-known supplier with comprehensive coverage in the life sciences industry and one of the world's largest producers of DNA synthesis. For nearly 30 years, Sangon Biotech has been synthesizing oligonucleotides for a variety of research applications. The number of scientific researchers wordwide using our primers has exceeded 70,000. We provide eight purification methods so that customers can choose the most suitable purification method according to their actual needs. We also specializes in modified oligonucleotides and we can provide more than 140 different types of oligo modifications.

Different Purification Methods
Purification Introduction Feature
Conventional purification methods HAP Purification by HAP resin purification column has a larger load than OPC and avoids the carryover of short fragments, increasing speed and purity. Stable quality with fast speed
PAGE Polyacrylamide gel electrophoresis (PAGE) based purification. Recommended for longer oligos. Salts removed. Majority of failure sequences removed. MS data provided. Stable quality with fast speed
ULTRAPAGE An upgrade of PAGE, the DNA was qualitatively analyzed with PAGE purification and mass spectrometry to ensure the accuracy of the recovered fragments. Stable quality with high rate of accuracy
HPLC High Performance Liquid Chromatography (HPLC) based purification. Recommended for shorter oligos. Salts are removed. Majority of failure sequences are removed. MS data can be provided. High degree of automation with high purity
HPLC_CE HPLC with Capillary Electrophoresis. Guaranteed minimum purity of 85% ensured. Recommended for shorter oligos. Salts are removed. Majority of failure sequences are removed. MS data can be provided. HPLC+CE with higher purity
Additional purification methods IE-HPLC Ion exchange (IE) HPLC separates and purifies full-length primers from truncated primers based on relative charge difference, which can effectively remove N-1 short fragments. It is suitable for most modifications, but not for thio, sulfhydryl and other modifications. Higher purity
2X HPLC Dual HPLC purification (reversed-phase, then ion exchanged), especially suitable for modified primers longer than 59 bases Higher purity
PAGE+HPLC For primers with high purity requirements, we offer PAGE+HPLC dual purification, i.e. one PAGE purification followed by another HPLC purification to further improve purity, especially for primers longer than 59 bases with modifications Higher purity
Different Purification Methods
Purification Purification Method
HAP PAGE ULTRAPAGE HPLC HPLC_CE (IVD)
IE-HPLC
2X HPLC
PAGE+HPLC
Normal PCR/RT-PCR
DNA sequencing
Gene synthesis
Multiple PCR/qPCR
Diagnostic PCR amplification
NGS
Subcloning, point mutation
Gene construction/ RNA interference
PCR production used for gene cloning, expression or genetic recombination
Antisense nucleic acid
Modify or mark primers/chemical or physical applications
IVD
“√” means recommended modificaton methods
Clean DNA Workshop
100% MS Detection
DNA Drying Room
CE Detection
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